Factors modifying creatine accumulation in human skeletal muscle
The ingestion of 5g of creatine (Cr) in solution will raise the plasma Cr concentration from ~40 umol/l to 600 ˆ 800 umol/l within 1 hr and plasma levels then decrease to close to basal over the subsequent 5 hrs (Harris et al. 1992, Green et al. 1996a). Repeating this procedure on four evenly spaced occasions each day for five days can increase the muscle total Cr (TCr) store by up to 40%. This increase is comprised of changes in both free Cr and phosphocreatine (PCr), with the magnitude of increase in the former being the largest (Harris et al. 1992, Greenhaff et al. 1994). The variation between individuals in the magnitude of muscle TCr increase is marked, with the extent of uptake being inversely related to the initial muscle TCr content (Harris et al. 1992, Greenhaff et al. 1994). The reasons for the large variation between subjects in the magnitude of Cr accumulation during supplementation are unknown and require further investigation.
The majority of muscle Cr accumulation occurs within the initial two days of loading and muscle Cr accumulation is saturated following five days of supplementation with 4 x 5g doses (Harris et al. 1992, Hultman et al. 1996). If Cr ingestion is stopped following loading, muscle Cr stores decline gradually and basal levels are reached after about four weeks (Febbraio et al. 1995, Hultman et al. 1996). Ingesting Cr at a rate of 3g per day will increase muscle Cr content but the time-course of change is slower, i.e. it takes 30 days to reach muscle TCr values similar to those observed after five days of 20g/day Cr ingestion (Hultman et al. 1996). Following loading, elevated muscle Cr stores can be maintained for at least one month by ingesting 2g of Cr per day in a single dose (Hultman et al. 1996). This maintains muscle Cr delivery at slightly above the rate of muscle Cr degradation to creatinine. Urinary creatinine output increases by about 20% which parallels the increase in muscle Cr content (Hultman et al. 1996).
Sub-maximal exercise performed prior to Cr ingestion can augment muscle Cr accumulation by about 10%, but again the variation in response is marked when comparing individuals (Harris et al. 1992).
Creatine plus carbohydrates
Muscle Cr accumulation can be substantially augmented by ingesting Cr in combination with large quantities of simple carbohydrates (Green et al. 1996b). This reduces the variation in responses between individuals and also outweighs any stimulatory effect exercise has on muscle Cr accumulation (Green et al. 1996a, 1996b). Muscle Cr accumulation is thought to be augmented as a result of insulin stimulating muscle sodium pump activity, and thereby sodium-dependent Cr transport. Recent evidence has demonstrated that it will require in the region of 100g of simple carbohydrates to be ingested to achieve an insulin-mediated stimulation of muscle Cr transport (Steenge et al. 1998). In practical terms, this will be difficult for individuals to achieve as the ingestion of such a large quantity of carbohydrate is at the limit of palatability. There are no data currently available to demonstrate the time-course of muscle Cr accumulation when Cr is ingested in the presence of large quantities of carbohydrate, i.e. how quickly is muscle Cr uptake saturated? This may be of significant practical importance.
A muscle Cr transport protein has recently been identified and it has been shown that its expression is down-regulated in rat skeletal muscle following six months of supra-physiological amounts of Cr supplementation (Guerrero-Ontiveros and Wallimann, 1998). Whether a similar response occurs in humans is unknown, as is the consequences of chronic Cr ingestion on the muscle Cr transport mechanism, i.e., why does the muscle become desensitised to Cr as a result of chronic ingestion? (For more on creatine and other ergogenics, see the middle pages of this issue).
Paul Greenhaff, School of Biomedical Sciences, University Medical School, Nottingham
References
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Green, A.L., Hultman, E., Macdonald, I.A., Sewell, D.A. and Greenhaff, P.L. (1996b).
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Steenge, G.R., Lambourne, J., Casey, A., Macdonald, I.A. & Greenhaff, P.L.
"The stimulatory effect of insulin on creatine accumulation in human skeletal muscle." Am J Physiol (1998), 275, E974-E979.